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Leave the vial at atmospheric pressure

The D-Phe stereochemistry and Nle substitution were specifically selected to resist enzymatic proteolysis, as established in the precursor work (Sawyer 1980, PMID 6777774)

The dose is increased in steps from 1.5mg up to a maintenance dose of 25mg

There is also substantial evidence that Pg can enter the liver and cause hepatic steatosis ( In contrast, although Fn can also induce intestinal inflammation and changes in the gut microbiota, its impact on TMAO production in healthy individuals under a normal diet is relatively weaker than Pg , possibly due to its less disruptive effect on the intestinal barrier