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Sections of deparaffinized recovered DRG tissue were blocked with 0.3% hydrogen peroxide for a period of 20 min and then incubated at 4 C for the entire night with anti-CD163 primary antibody supplied by GeneTex, CA, USA (GTX35247, 1:100) as well as anti-CD86 primary antibody provided by Bioss, USA (bs-1035R, 1:150)

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The severity of obesity is categorized as Class I (BMI 3034.9 kg/m), Class II (3539.9 kg/m), and Class III (40 kg/m), with higher classes conferring progressively greater morbidity risk[2]