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When there is a suspicion of symptomatic B12 deficiency it is important to measure the B12 serum determination (in addition to excluding other diseases, such as thyroid diseases and Lyme), even if anaemia has not been diagnosed

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(However, note that when injecting intramuscularly or subcutaneously, any trace preservative is generally well tolerated

was recorded at 450 nm using a TECAN GENios plate reader