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RNA interference and EM studies hinted that this was functionally relevant since DDX3X depletion led to an accumulation of enveloped perinuclear virions in nuclear herniations similar to those reported for HSV-1 or PRV mutants lacking the viral pU S 3 kinase or those that simulate constitutively phosphorylated pU L 31 59

A further exploration of metabolic changes in IIH utilised ultrahigh-performance liquid chromatography-mass spectrometry to perform an untargeted metabolomic analysis in serum and CSF of patients with IIH and healthy controls [23]

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