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By treating metabolically active adipocytes and hepatocytes with 5-amino-1-methylquinolinium, researchers can precisely inhibit intracellular NNMT activity, monitor changes in the nicotinamide pathway and NAD+ levels, and analyze effects on energy metabolism

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They are viewed as an intermediate model between 2D monolayers and animal models, as they preserve key features of tumors, especially the response to drug treatments ( Treating the Th- MYCN spheroids with the DPI-MitoQ combination showed a strong synergistic effect