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Subsequently, the Recombinant Human Dynamin-1-like protein (DRP1, #CSB-EP007078HU, Cusabio Biotech, Wuhan, China) was diluted to 10 g/mL in PBS and injected at 10 L/min for an appropriate duration

Green, MD , professor of medicine at Duke University School of Medicine: While losing weight, our brain has kind of a weight-happy place and when we move away from that, our brain is unhappy and alters hormone levels to counteract it, so our body makes it more difficult to continue to lose weight once weve started losing and unfortunately, it tends to make us regain the weight back. This is supported by studies showing that the hunger hormone ghrelin increases with weight loss, and leptin which helps prevent hunger and regulate energy balance decreases with weight loss [18]