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Another study with CuCl 2 treated Hep G2 cell line demonstrated that mitochondrial toxicity is an intermediate-level event associated with copper toxicity to Hep G2 cells while lysosomal damage is the early event and plasma damage a late feature associated with copper toxicity 34

Apart from that, Glutathione plays major roles in different cellular compartments

However, the lack of a significant difference in ROS between the combination group and the GSH monotherapy group indicated that ROS may not have a significant correlation with this synergistic effect, and the specific mechanism needs further research

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