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[DOI] [PMC free article] [PubMed] [Google Scholar] Carvalho-Freitas MI, Anselmo-Franci JA, Maiorka PC, Palermo-Neto J, Felicio LF (2011) Prolactin differentially modulates the macrophage activity of lactating rats: possible role of reproductive experience

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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit