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Aligned with earlier findings, semaglutide treatment at dosages from 0.5 mg to 2 mg weekly demonstrated variable efficacy, from merely preventing further weight gain in Patient 1 to achieving weight loss of up to 14.4% and 11% relative to baseline, in Patient 2 and Patient 3, respectively

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The primary analytical challenges in semaglutide characterization arise from several structural features, including: Acylated lysine residue Fatty acid side chain Spacer/linker chemistry Multiple formulation-associated variants These modifications create substantial analytical heterogeneity that can complicate LC-MS workflows, chromatographic separation, ionization efficiency, and fragmentation analysis