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All positively stained cells were counted and the % Nissl positive neuron was calculated by dividing the number of positive neurons by the total number of neurons in each cell [1]

A 12-week randomized clinical trial cited across NIH/PMC-indexed research on GHK-Cu's regenerative actions found significant anti-aging benefits from a GHK-Cu facial cream applied twice daily, including reduced fine lines, coarse wrinkles and mottled pigmentation, increased skin density and thickness, and strongly stimulated keratinocyte proliferation

This can be caused by factors such as the size of the needle used, poor visibility during the procedure, and the injection technique employed