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10.1038/s41467-022-29905-1 117 KoppulaP.ZhangY.ZhuangL.GanB

Written by Ali Rza Akn Microbiome Scientist, Author & Founder of Next-Microbiome Ali Rza Akn is a microbiome scientist with nearly 30 years of experience in translational biotechnology, systems biology, and applied microbiome research, spanning discovery, preclinical development, and clinical-stage translation

In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione

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