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The increased glucose (25 mM) was used to inhibit -cell glucagon secretion to diminish glucagon levels to basal levels to improve the dynamic range of the assay and thus effectively unmask the role of ligand-mediated glucagon secretion as described earlier 45

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Zimmermann MB, Aeberli I

The clientized S tail has broad selectivity for WD40 and WD40 Prior work revealed that the wild-type S heptapeptide does not interact strongly with WD40 43 , even though there is complete conservation of the basic cluster in WD40 and WD40 domains 61,62