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Cells were harvested by centrifugation (800 g for 15 minutes), and the resulting pellet was resuspended in 50 mL lysis buffer (10 mM Hepes, pH 7.5, 400 mM NaCl, 1.5 mM DTT) supplemented with 1 protease inhibitor cocktail (Roche)

Correlations between inflammatory markers and OS parameters suggest that PASC individuals experience significant oxidative stress, which may exacerbate disease complications

Vitamin D3 helps enhance calcium absorption by regulating concentrations of calcium and phosphorus in the body to provide essential support for bones and teeth

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