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PC knockdown PC was suppressed in metM-Wnt lung cells and E0771 cells by lentiviral transduction for 48 h, using particles generated by transfection of HEK293T cells with psPAX2 (Addgene), pMD2.G (Addgene), and Pcx-targeting shRNAs

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Allicin breaks down in vitro to form a variety of fat-soluble organosulfur compounds ( Figure 2 ), including diallyl trisulfide (DATS), diallyl disulfide (DADS), and diallyl sulfide (DAS), or in the presence of oil or organic solvents, ajoene and vinyldithiins (6)