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Peripheral venous blood samples were obtained by venipuncture at the antecubital fossa (Vacutainer Blood Collection Set, BD Vacutainer, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) using 3 mL K2EDTA Vacuettes and Serum Sep Clot Activator vacutainers (Greiner Bio-One Gmbh, Kremsmuenster, Austria)

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