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Isolated islets were cultured in RPMI 1640 medium (Hyclone, South Logan, UT, USA) with 11.1 mM glucose (Solarbio, Beijing, China) and 10% fetal bovine serum (Gibco, Grand Island, NY, USA) supplemented with 1% penicillin and streptomycin under 37 C and 5% CO 2 conditions

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Step-by-Step Reconstitution Protocols for CJC-1295 Research Peptide in Lab Settings Research Notice: This article covers research on CJC-1295 without DAC research peptide and CJC-1295 with DAC research peptide available from Palmetto Peptides for laboratory use only