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The NRF2 pathway is activated in the setting of acute inflammation and functions to reduce excessive tissue damage by tampering the acute cytotoxic effects of the innate immune response

The HRP reaction was stopped by the addition of 100 l of 1 M phosphoric acid, and the absorbance at a wavelength of 450 nm was measured using a microplate reader (TriStar LB942)

Moreover, the acidic assay conditions (pH 3.6) can precipitate proteins (e.g., milk casein) and generate pro-oxidant Fe 2+ ions, which may propagate radical chain reactions (Pulido et al., 2000