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GSH was measured by HPLC (LC-10AT, Shimadzu, Japan) using a WondalSil C18 column (GL Sciences Inc., Japan) and a UV detector (SPD-20A, Shimadzu, Japan) at 210 nm

It is important to be aware of these potential side effects and the long term risks that could be caused by the solution itself or the incorrect administration of the solution

Briefly, tissues were homogenized in 500 L of RNAiso plus, with total RNA separated by mixing with 100 L of chloroform, and isolated by isopropanol precipitation

Although a decrease in the intensity of infection was observed, the infection rates of the MB groups were similar to the control groups