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VIGS assays were performed as previously described 50

For compounds requiring acetic acid acidification for solubility and including some hydrophobic peptides , Bacteriostatic Water may need to be acidified with sterile acetic acid to achieve the required pH for dissolution before addition to the lyophilised compound

Taken together, these findings demonstrate that uridine supplementation enhances cytoplasmic maturation in aged oocytes by restoring the dynamics of CG and ovastacin, thereby improving fertilization outcomes and subsequent embryonic development

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