glutathione and smoking with Cofactors — Seeking Health Suggested role of GSTO (glutathione

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Vigorous agitation introduces foam and can destabilize protein and peptide solutions

The supernatants were centrifuged at 10,000 g for 15 min at 4C, and the mitochondrial pellets were dissolved in sodium dodecyl sulfate (SDS) sample buffer, subjected to 15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and analyzed by immunoblotting with monoclonal antibodies against cytochrome c and Cox IV