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Demographic characteristics and clinical severity assessed using the Childhood Autism Rating Scale (CARS) were not re-analyzed in the current study and are referenced solely for cohort description and verification, in line with transparent reporting practices

Subsequently, the samples were permeabilized with 0.3% Triton X-100 in PBS at room temperature for 10 min

Thus, endothelial OS is both a driver and consequence of HTN, making it a pivotal target for interventions aimed at breaking the cycle of vascular dysfunction