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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

5UTR refers to those GpGs within the 5 untranslated region, between the TSS and the ATG start site

The results were highly significant: the WPI group demonstrated a massive 46.6% increase in working memory and a 46.7% improvement in total memory scores, vastly outperforming the control group

Inhibition of LTR signalling activates WNT-induced regeneration in lung