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4: Mass Spectrometry Challenges in Semaglutide Characterization Semaglutide is considered one of the most analytically complex GLP-1 therapeutics due to its engineered peptide structure and lipid conjugation

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The lid was opened to volatilize off the excess ethanol, which is then added to diethyl pyrocarbonate water (NR0001, Regen, China) to be stored in 80C