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After binding for 10 min at 37C and separation of the bound complexes by centrifugation, the amounts of bound and free GST were determined by the Lowry method [29]

Methionine is an essential amino acid and a precursor for S-adenosylmethionine (SAM), a vital methyl donor in various biochemical reactions (19, 20, 25, 71, 76)

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