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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

Cell death was quantified by annexin V/propidium iodide (PI) staining, with z-VAD-FMK (a pan-caspase inhibitor) used to assess the involvement of caspase-dependent apoptosis after 6 and 24 h of treatment (Fig

If you are currently using Saxenda daily and wish to switch to Wegovy, our clinicians will advise you how best to switch medications

Only research-grade or anti-doping labs use equipment sensitive enough to identify them