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Briefly, INS-1E cells were maintained in RPMI 1640 medium (Gibco) supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin/streptomycin and 50 M 2-mercaptoethanol

While preservatives can help prevent bacterial growth, they do not stop the primary issue: thermal degradation of the peptide bonds

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But the reason providers are increasingly prescribing them together is because they work on different but complementary repair systems, and they do not interfere with each other