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The soluble clarified fraction was recovered by centrifuging at 75,000 g for 20 min at 4 C and applied to a Superdex 200 26/600 column (Cytiva) for SEC, which was run as above

In irradiated 3T3 fibroblasts, GHK treatment decreased the expression of p21, a key marker of cellular senescence (Figure 2A)

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