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The Q-Exactive Plus Mass Spectrometer (Thermo Fisher Scientific) was equipped with electrospray ionization (ESI) operated in the positive mode: ESI spray voltage +3.8 kV, sheath gas (N 2 ) 20 a.u., auxiliary gas (N 2 ) 5 a.u., capillary temperature 320 C, and mass resolution 70,000 at m/z 400

As per the data from the University of California Health Data Warehouse, Ozempic (semaglutide, a GLP-1 RA) users have increased from 569 to 7667 to 22,891 in 2019, 2020, and 2022, respectively [5]

This decrease in glutathione's protective function can lead to increased oxidative stress, potentially negatively affecting the aging process

Dietary restriction transforms the mammalian protein persulfidome in a tissue-specific and cystathionine gamma-lyase-dependent manner