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Evaluation of N-glycosylation of secreted proteins N-glycosylation was evaluated by comparative WBs under basal conditions and after digestion with neuraminidase ((2 3, 6, 8, 9)) from Arthrobacter ureafaciens , Sigma-Aldrich), following the manufacturers instructions, and with N-glycosylase F (PNGase F, Sigma-Aldrich, Madrid, Spain)

The result shows that the viability of the cells treated with TPE-DCV is almost unchanged as compared to the control untreated cells, suggesting the dye at the tested concentration range would not interfere with the physiology or proliferation of the cells

HSS Journal, Article 15563316251355551

SIRT3, as a deacetylase in mitochondria, modulates the transcription of mitochondrial in response to oxidative stress