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After de-paraffinization and rehydration, sections underwent a heat-induced epitope retrieval by microwave heating in 0.1 M TRIS hydrochloride (HCl) (pH 9.0) for 15 min

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Despite recently becoming a buzzy topic in longevity circles, glutathione has actually been studied for decades because of the role it plays in antioxidant defense, cellular protection, detoxification, and oxidative stress regulation throughout the body

Changes in gene expression levels of glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx1), glutathione reductase (GR) and glutathione synthetase (GS) genes were analyzed by real-time qPCR on ABI PRISM 7500 (Applied Biosystems)