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Patients should be counseled about the risk of dehydration and potential acute kidney injury with severe gastrointestinal symptoms, and the need to temporarily hold medication and seek care if severe vomiting or diarrhea occurs

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For people who were not given support, they didn't meet with a personal trainer, they didn't get a registered dietician to help them

After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)