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Published pharmaceutical literature describes the physiological response to injected endotoxins: fever (pyrogenic response), injection-site inflammation, and systemic inflammatory markers at higher exposure levels

The ASG-10 cell line was previously made by our lab (Gjessing et al

Commonly measured antioxidant enzymes include glutathione (GSH), total antioxidant capacity (TAC), glutathione peroxidase (GPx), and superoxide dismutase (SOD), while malondialdehyde (MDA) is often used as a marker of oxidative stress

Isopropyl -D-1-thiogalactopyranoside (Fermentas, Germany) was added till a final concentration of 1.0 mM in order to induce the bacterial cells