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The percentage of viable and apoptotic cells was evaluated by flow cytometry (Accuri C6, BD Biosciences)

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Wnt/-catenin pathway as a biomarker in the diagnosis of tumour-related diseases Wnt/-catenin pathway in the cell-free DNA (cfDNA) test for CRC The traditional diagnosis of CRC has many limitations, 243,244,245 such as low sensitivity, poor specificity, and painful and traumatic procedures