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Nitric oxide synthases are very complex enzymes, employing five redox cofactors: NADPH, FAD, FMN, heme, and tetrahydrobiopterin (H 4 B

We acknowledge Diamond Light Source for access and support of the cryo-EM facilities at the UKs national Electron Bio-imaging Centre (eBIC), instrument eBIC Titan Krios IV [under proposal EM20223-37], funded by the Wellcome Trust, MRC and BBRSC

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Then allowing the vial to sit undisturbed for 35 minutes while the peptide dissolves passively