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In humans, an improvement in psychomotor state and oxidative stress markers (GPX, SOD) has been observed following the ingestion of 5 mg/kg caffeine in athletes participating in an endurance race after 26 h of sleep deprivation [79]

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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)