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Briefly, mice were anesthetized with ketamine/xylazine and do not suffer pain from this terminal procedure

State Appearance after recon Storage Clear, colorless solution Notes Cloudy or particulate solutions should not be used

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All glassware used for sample storage and preparation were cleaned by washing with soap, rinsing with DI and fired at 400 C overnight to ignite any interfering organic residues on surfaces that may have interfered with quantitative analysis