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For BAC water, that means sterile bacteriostatic water produced to USP standards, not a non-sterile alternative

In practice, these arms operate simultaneously: biosynthesis is tuned to folding capacity, and degradation capacity feeds back to biosynthesis through stress signaling and metabolic cues

berghei transfections were accomplished by electroporation of purified schizonts using the Amaxa Nucleofector Technology (Lonza) as described previously ( pbgst - ko 1A and 1B , containing the tgdhfr/ts selectable marker, were selected with pyrimethamine as previously described ( pbgst - ko 2A and 2B , which contains the hdhfr selectable marker, were selected with WR99210 ( pbgst-ko 2A and 2B were done in parallel with a previously described plasmid targeting the pbmrp as a transfection control (Rijpma et al., 2016)

Salehi, B., et al