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Cells were cultivated in EBM2 medium (Lonza, Basel, Switzerland) containing 10% FCS, 1% penicillin/streptomycin solution and 1% amphotericin B (both from Sigma-Aldrich, Taufkirchen, Germany) in dishes coated with collagen A (20 g/ml) (Biochrom, Berlin, Germany) at 37 C and 7.5% CO 2

51 Upregulating SIRT3 expression and activation of the extracellular signal-regulated kinase-Yes-associated protein (ERK-Yap) signaling pathway, as well as the CREB/adiponectin axis may also be involved in the protection of mitochondria by GLP-1

All peptides at MDTW are sourced from licensed compounding pharmacies based in the USA

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