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To purify noncyclic K29-Ub4, the reaction solution was mixed with four volumes of 50 mM ammonium acetate buffer (pH 4.5) and applied to a Resource S cation-exchange column (Cytiva) pre-equilibrated with the same buffer

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Angiotensin II (Ang II), a key component of RAAS, is known to stimulate the production of ROS, particularly in vascular smooth muscle cells and endothelial cells [80]

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Because the risks are no longer theoretical - theyre behavioural

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