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A Initial injection Conditions Stationary phase: Kromasil 100-10-C4 Mobile phase solvent: acetonitrile Mobile phase salt: ammonium acetate Pool analysis, 95% purity Conditions Phase: Kromasil 100-3.5-C4 Eluent: acetonitrile / water Eluent salt: trifluoroacetic acid B After 30 sequential prep injections Conditions Stationary phase: Kromasil 100-10-C4 Mobile phase solvent: acetonitrile Mobile phase salt: ammonium acetate Pool analysis, 77% purity Conditions Phase: Kromasil 100-3.5-C4 Eluent: acetonitrile / water Eluent salt: trifluoroacetic acid After 30 preparative injections, column efficiency is impaired resulting in lower purity in extracted fractions

Then some areas of bronzing where the skin can darken, dry and slough off may occur for 1-2 days