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A pure RBC suspension can reliably be achieved by density-gradient centrifugation (2500 RPM, 15 min, RT)

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We showed that mice fed a high-fat diet (HFD) for 12 weeks developed hepatic steatosis and liver injuries, which were associated with not only TNF overproduction but also hepatic glutathione dysregulation, characterized by GSH reduction and GSSG elevation

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Autonomic and Neurologic Symptoms The authors note that atypical clinical presentations of Lyme disease can occur, including gastrointestinal manifestations related to autonomic dysfunction , as was seen in this case

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