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Escalation typically proceeds: 2.5mg weeks 1-4, 5mg weeks 5-8, 7.5mg weeks 9-12, with potential increases to 10mg, 12.5mg, or 15mg
Individual fish were divided using a razor blade into 0.5 cm sections and added to a 40 mL glass tube along with 0.1 mL of DI water (18 M-cm Milli-Q, EMD Millipore, Burlington, MA) whereupon tissues were homogenized into a paste using an Omni TH (Omni Int., Kennesaw, GA) tissue homogenizer
GHK-Cu (glycyl-L-histidyl-L-lysine copper) is a naturally occurring peptide in the human body that binds copper ions, an essential element for tissue repair and cellular function
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