Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
General Information: Glutathione is an antioxidant thats made up of three amino acidscysteine, glycine, and glutamine
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Your body then releases glutathione (and other antioxidants) to scavenge for free radicals and quench their effect, depleting overall levels if your body can't keep up with the glutathione demand