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High abdominal pressure at 25, 30, 40, or 50 mmHg was maintained until sacrifice at 60 min (25 mmHg), 30 min (30 mmHg, 40 mmHg), or 15 min (50 mmHg)

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Understanding the molecular mechanisms by which pyocyanin manipulates host cell functions are vital for developing targeted therapeutic approaches to mitigate its harmful effects and improve patient outcomes