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Replacing the sulfonic acid at position R 1 with a sulfonamide group ( 33 ) resulted in a drastic decrease in solubility of the fragment with a concomitant reduction of binding to TRF1 TRFH by CPMG, whilst the dimethyl sulfonamide 35 or the even bigger benzyl sulfonamide 36 showed an even poorer solubility and a loss of TRF1 TRFH binding (Table 1)

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For a visual walkthrough of this same process applied to other peptides, our guide to mixing peptides with bac water covers the universal technique