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The link to stress regulation is part of why DSIP is sometimes discussed alongside both sleep and resilience

Quantitative real-time PCR Total RNA was isolated from tissue or cells using Qiazol and RNeasy mini kit (Qiagen, Venlo, Netherlands) according to manufacturers instructions and with 15 min on-column DNase digestion (Qiagen, Venlo, Netherlands)

AAM, BJP, and AS performed literature searches and selected the studies and reviews discussed in the manuscript

Other risk factors include certain gastrointestinal conditions, autoimmune conditions such as pernicious anaemia, long-term use of some medications, and increasing age