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Fragment 176-191 is used in in vitro research examining adipocyte biology and lipid metabolism regulation at the cellular level, including interactions with beta-3 adrenergic receptor-related signalling pathways in adipocyte cell line models
The membranes were sequentially probed with the primary antibodies (1:5000) at 4 C overnight and corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000, Pierce at room temperature for 1 h)
Manufacturing processes matter significantly
It is extracted from Chinotto a fruit-bearing tree native to Southern China (hence its name) which was imported in Italy most probably around the VI and VII century A
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