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Surgical jawline restoration becomes the recommended approach when skin laxity exceeds what fillers can address, when jowling requires tissue repositioning rather than volume alone, or when patients desire permanent results rather than ongoing maintenance treatments

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Plates were incubated for 15 min in a Spark 20M (Tecan) plate reader at 30 C and A 340nm was measured every 26 s with a 5 s shaking interval between measurements at 180 rpm using a 3 mm shaking diameter