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Following two consecutive 5-min washing steps in PBS, sections were blocked with 10% (vol./vol.) normal goat serum constituted in low protein blocking solution (eBioscience, San Diego, CA, USA) for 1 h at 37 C, followed by incubation with rabbit anti-GPX1 (1:120, diluted in antibody diluent from Cell Signaling Technology, Danvers, MA, USA) for 1618 h at 4 C

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