Primary antibodies for western blotting Immunoblotting was performed with rabbit polyclonal anti-GCL (1:1,000) 5 , rabbit polyclonal anti-GPX1 (1:1,000) (ab59546, Abcam, Cambridge, UK), rabbit polyclonal anti-GFP (1:1,000) (ab290, Abcam), mouse monoclonal anti-GSS (1:1,000) (Sc-166882, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-GSR (Sc-133159, Santa Cruz Biotechnologies), mouse monoclonal anti-SOD2 (1:2,000) (ab16,956, Abcam), rabbit polyclonal anti-VDAC (1:1,000) (Calbiochem, Darmstadt, Germany) or mouse monoclonal anti-GAPDH (1:40,000) (glyceraldehyde-3-phosphate dehydrogenase
What makes GHK-Cu remarkable is the scope of its biological activity
These deficiencies might be caused by many reasons, such as anorexia, lactation, stress, or overexertion
The researchers further speculated that the peptide could have influenced umbilical vein endothelial cell proliferation (HUVECs)